Class 11 Zoology simulations · Biomolecules

Enzyme action and competitive inhibition

Raise the substrate concentration and see the reaction rate level off. Add a competitive inhibitor and compare.

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What to notice

A competitive inhibitor resembles the substrate and competes for the active site; enough substrate can outcompete it.

Key relation

v = V_max[S] / (K_m + [S]) ; competitive: K_m(app) = K_m(1 + [I]/K_i)

Try this

Add inhibitor, then raise [S] a lot. Does V_max change?

What you can change

• Substrate concentration [S]: 0 to 50 mM
• Michaelis constant K_m: 1 to 20 mM
• Maximum rate V_max: 10 to 100 μmol/min
• Competitive inhibitor [I]: 0 to 20 mM

More in Biomolecules

• Temperature and pH affect enzymes
• Levels of protein structure

See all Class 11 Zoology simulations or every Physica simulation.