Class 12 Zoology simulations · Biotechnology: Principles and Processes

Agarose gel electrophoresis

Load DNA fragments into wells and run the gel. Negatively charged DNA moves towards the anode; smaller fragments move farther.

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What to notice

Bands are seen after staining with ethidium bromide and UV light; they can be cut out (elution) and used for cloning.

Key relation

Distance moved ∝ log of (1 / fragment size), roughly

Try this

Increase the run time. Do small fragments run off the gel first?

What you can change

• Run time: 0 to 60 min
• Voltage: 50 to 150 V
• Your sample: fragment size: 200 to 9000 bp

More in Biotechnology: Principles and Processes

• Polymerase chain reaction (PCR)
• Making recombinant DNA

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